Lab Report 2 Essay Example | Topics and Well Written Essays - 2250 words

Lab Report 2 - Essay Example Short tandem repeat or STR is polymorphic DNA loci present on chromosome containing repeated nucleotide sequence having two to seven nucleotides repeat. The number of repetition of this nucleotide is unique to particular individual and hence can be use as signature for that particular individual. After realization of STR profiling assay many companies have come out with commercial kit for easy analysis and reproducibility. AmplSTR COfiler PCR kit developed by applied biosystems is one of them. It amplifies 6 somatic STR loci along with one sex determination or amelogenin STR loci. Kit also includes positive and negative standard and amplified STR directly load in to 3100 Avant genetic analyzer and STR profile will be obtained by given software. In case of unknown criminal STR profile is matched with CODIS database to search for probable suspect. Crimes and criminal has become an integrated part of human society and the constant evolution of criminal procedure and sophistication used by criminals makes it huge task for crime investigators to identified criminals. The science used in crime investigation is known as forensic science which utilizes various scientific methodologies to identify the criminals and establish the links between crime and criminals. The biggest challenges in front of forensic experts includes very little sample at crime site, mixing up of evidences, and many in many cases completely burn victims etc. Development of DNA based identification system has revolutionized the way forensic science was operated. The biggest advantage of DNA based techniques is the requirement of very small amount of biological samples which includes blood, hair, semen or any body parts etc. Similarly, one can obtain DNA sample from highly decomposed or burned victim’s bodies. There are several reports where DNA based tec hniques are employed to